Device

Part:BBa_K1698004:Design

Designed by: Brandon Malone   Group: iGEM15_Cork_Ireland   (2015-09-18)


BaseHunter : Activated to become detector for 24bp target sequence on Mycobacterium genomes


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 31
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1


Design Notes

For the detailed design of this sequence, we just had to make sure that the complementary target would not form hairpin structures or dimerize and would have a rather low GC content


Source

Part is non-coding. It was prepared by sequencing and ligation into PSB1C3

References